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Antibodies used in the presented studies.
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Antibodies used in the presented studies.
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Antibodies used in the presented studies.
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Antibodies used in the presented studies.
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Antibodies used in the presented studies.
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( A ) Schematic showing steps involved in the creation of BRITER Cell Line. ( B ) Schematic showing critical genetic components of BRITER Cell Line. ( C ) BMP2 dependent FFLuc activity of BRITER Cell Line under four different conditions, namely “−BMP. −4-OHT”, “−BMP, +4-OHT”, “+BMP, +4-OHT” and “+BMP, −4-OHT”. ( D <t>)</t> <t>Anti-PSMAD</t> 1/5/8 immunofluorescence in BRITER cell line under four different conditions: ( a ) −BMP−4-OHT, ( b ) −BMP+4-OHT, ( c ) +BMP+4-OHT and ( d ) +BMP−4-OHT. Scale bar 100 µm. ( E ) Quantification of Anti-PSMAD 1/5/8 immunofluorescence by Image J. ( F ) Western blot analysis of BRITER cell extracts cultured under indicated conditions with PSMAD 1/5/8 antibody. β-tubulin antibody has been used as loading control. Data shown are means ± SEM of three independent experiments carried out in triplicates.
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( A ) Schematic showing steps involved in the creation of BRITER Cell Line. ( B ) Schematic showing critical genetic components of BRITER Cell Line. ( C ) BMP2 dependent FFLuc activity of BRITER Cell Line under four different conditions, namely “−BMP. −4-OHT”, “−BMP, +4-OHT”, “+BMP, +4-OHT” and “+BMP, −4-OHT”. ( D <t>)</t> <t>Anti-PSMAD</t> 1/5/8 immunofluorescence in BRITER cell line under four different conditions: ( a ) −BMP−4-OHT, ( b ) −BMP+4-OHT, ( c ) +BMP+4-OHT and ( d ) +BMP−4-OHT. Scale bar 100 µm. ( E ) Quantification of Anti-PSMAD 1/5/8 immunofluorescence by Image J. ( F ) Western blot analysis of BRITER cell extracts cultured under indicated conditions with PSMAD 1/5/8 antibody. β-tubulin antibody has been used as loading control. Data shown are means ± SEM of three independent experiments carried out in triplicates.
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( A ) Schematic showing steps involved in the creation of BRITER Cell Line. ( B ) Schematic showing critical genetic components of BRITER Cell Line. ( C ) BMP2 dependent FFLuc activity of BRITER Cell Line under four different conditions, namely “−BMP. −4-OHT”, “−BMP, +4-OHT”, “+BMP, +4-OHT” and “+BMP, −4-OHT”. ( D <t>)</t> <t>Anti-PSMAD</t> 1/5/8 immunofluorescence in BRITER cell line under four different conditions: ( a ) −BMP−4-OHT, ( b ) −BMP+4-OHT, ( c ) +BMP+4-OHT and ( d ) +BMP−4-OHT. Scale bar 100 µm. ( E ) Quantification of Anti-PSMAD 1/5/8 immunofluorescence by Image J. ( F ) Western blot analysis of BRITER cell extracts cultured under indicated conditions with PSMAD 1/5/8 antibody. β-tubulin antibody has been used as loading control. Data shown are means ± SEM of three independent experiments carried out in triplicates.
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( A ) Schematic showing steps involved in the creation of BRITER Cell Line. ( B ) Schematic showing critical genetic components of BRITER Cell Line. ( C ) BMP2 dependent FFLuc activity of BRITER Cell Line under four different conditions, namely “−BMP. −4-OHT”, “−BMP, +4-OHT”, “+BMP, +4-OHT” and “+BMP, −4-OHT”. ( D <t>)</t> <t>Anti-PSMAD</t> 1/5/8 immunofluorescence in BRITER cell line under four different conditions: ( a ) −BMP−4-OHT, ( b ) −BMP+4-OHT, ( c ) +BMP+4-OHT and ( d ) +BMP−4-OHT. Scale bar 100 µm. ( E ) Quantification of Anti-PSMAD 1/5/8 immunofluorescence by Image J. ( F ) Western blot analysis of BRITER cell extracts cultured under indicated conditions with PSMAD 1/5/8 antibody. β-tubulin antibody has been used as loading control. Data shown are means ± SEM of three independent experiments carried out in triplicates.
Anti Psmad/1/5/8, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Antibodies used in the presented studies.

Journal: eLife

Article Title: The hepcidin regulator erythroferrone is a new member of the erythropoiesis-iron-bone circuitry

doi: 10.7554/eLife.68217

Figure Lengend Snippet: Antibodies used in the presented studies.

Article Snippet: pSMAD 1/5/8 (monoclonal) , Cell signaling , 9516 , 1:1000 , BSA , Rabbit.

Techniques:

( A ) Schematic showing steps involved in the creation of BRITER Cell Line. ( B ) Schematic showing critical genetic components of BRITER Cell Line. ( C ) BMP2 dependent FFLuc activity of BRITER Cell Line under four different conditions, namely “−BMP. −4-OHT”, “−BMP, +4-OHT”, “+BMP, +4-OHT” and “+BMP, −4-OHT”. ( D ) Anti-PSMAD 1/5/8 immunofluorescence in BRITER cell line under four different conditions: ( a ) −BMP−4-OHT, ( b ) −BMP+4-OHT, ( c ) +BMP+4-OHT and ( d ) +BMP−4-OHT. Scale bar 100 µm. ( E ) Quantification of Anti-PSMAD 1/5/8 immunofluorescence by Image J. ( F ) Western blot analysis of BRITER cell extracts cultured under indicated conditions with PSMAD 1/5/8 antibody. β-tubulin antibody has been used as loading control. Data shown are means ± SEM of three independent experiments carried out in triplicates.

Journal: PLoS ONE

Article Title: BRITER: A BMP Responsive Osteoblast Reporter Cell Line

doi: 10.1371/journal.pone.0037134

Figure Lengend Snippet: ( A ) Schematic showing steps involved in the creation of BRITER Cell Line. ( B ) Schematic showing critical genetic components of BRITER Cell Line. ( C ) BMP2 dependent FFLuc activity of BRITER Cell Line under four different conditions, namely “−BMP. −4-OHT”, “−BMP, +4-OHT”, “+BMP, +4-OHT” and “+BMP, −4-OHT”. ( D ) Anti-PSMAD 1/5/8 immunofluorescence in BRITER cell line under four different conditions: ( a ) −BMP−4-OHT, ( b ) −BMP+4-OHT, ( c ) +BMP+4-OHT and ( d ) +BMP−4-OHT. Scale bar 100 µm. ( E ) Quantification of Anti-PSMAD 1/5/8 immunofluorescence by Image J. ( F ) Western blot analysis of BRITER cell extracts cultured under indicated conditions with PSMAD 1/5/8 antibody. β-tubulin antibody has been used as loading control. Data shown are means ± SEM of three independent experiments carried out in triplicates.

Article Snippet: PSMAD 1/5/8 was detected by incubating the membrane with antibody against PSMAD 1/5/8 (Cell Signaling, U.S.A.) at 1∶1000 dilution for 4 hours at room temperature followed by incubation with 1∶2000 dilution of anti-rabbit immunoglobulin coupled to Horse Radish Peroxidase (Jackson Immunoresearch).

Techniques: Activity Assay, Immunofluorescence, Western Blot, Cell Culture, Control