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Image Search Results
Journal: eLife
Article Title: The hepcidin regulator erythroferrone is a new member of the erythropoiesis-iron-bone circuitry
doi: 10.7554/eLife.68217
Figure Lengend Snippet: Antibodies used in the presented studies.
Article Snippet:
Techniques:
Journal: PLoS ONE
Article Title: BRITER: A BMP Responsive Osteoblast Reporter Cell Line
doi: 10.1371/journal.pone.0037134
Figure Lengend Snippet: ( A ) Schematic showing steps involved in the creation of BRITER Cell Line. ( B ) Schematic showing critical genetic components of BRITER Cell Line. ( C ) BMP2 dependent FFLuc activity of BRITER Cell Line under four different conditions, namely “−BMP. −4-OHT”, “−BMP, +4-OHT”, “+BMP, +4-OHT” and “+BMP, −4-OHT”. ( D ) Anti-PSMAD 1/5/8 immunofluorescence in BRITER cell line under four different conditions: ( a ) −BMP−4-OHT, ( b ) −BMP+4-OHT, ( c ) +BMP+4-OHT and ( d ) +BMP−4-OHT. Scale bar 100 µm. ( E ) Quantification of Anti-PSMAD 1/5/8 immunofluorescence by Image J. ( F ) Western blot analysis of BRITER cell extracts cultured under indicated conditions with PSMAD 1/5/8 antibody. β-tubulin antibody has been used as loading control. Data shown are means ± SEM of three independent experiments carried out in triplicates.
Article Snippet: PSMAD 1/5/8 was detected by incubating the membrane with
Techniques: Activity Assay, Immunofluorescence, Western Blot, Cell Culture, Control